Fluorescent Dyes for Bioconjugation and Detection Applications
Fluorescent Dyes: Excitation 500–600 nm
Orange and red fluorophores for advanced labeling applications
This range features non-activated and amine-reactive dyes like TAMRA, ROX, and JOE, with excitation in the 500–600 nm range. These dyes are ideal for bioconjugation, FRET, and fluorescence detection in imaging and flow cytometry.
Their bright signal and spectral properties make them well-suited for multiplexing and high-sensitivity assays.
For other spectral options, see also:
Excitation 350–500 nm – Green-fluorescent dyes including fluorescein and FITC derivatives
Excitation 500–600 nm – Orange and red-fluorescent rhodamines and TAMRA-based labels
Excitation 600–700 nm – Far-red fluorescent dyes such as ROX and X-rhodamine
Excitation 500–600 nm
6-Carboxy-X-rhodamine NHS ester, single isomer (6-ROX-SE)
Amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 584 nm, Em: 599 nm).
5-Carboxytetramethylrhodamine, single isomer (5-TAMRA)
Classic FRET acceptor, non-activated (Abs: 548 nm, Em: 570 nm).
5-Carboxytetramethylrhodamine NHS ester, single isomer (5-TAMRA-SE)
Amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm).
5-Carboxy-X-rhodamine, single isomer (5-ROX)
Non-activated red fluorescent dye label (Abs: 576 nm, Em: 601 nm).
6-Carboxytetramethylrhodamine NHS ester, single isomer (6-TAMRA-SE)
Amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm).
6-Carboxytetramethylrhodamine, single isomer (6-TAMRA)
Classic FRET acceptor, non-activated (Abs: 549 nm, Em: 572 nm).
5/6-Carboxytetramethylrhodamine NHS ester, mixed isomers (5/6-TAMRA-SE)
Amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm).
5/6-Carboxy-Erythrosin B
Non-activated singlet-oxygen generator and dye marker for labeling of biomolecules (Abs: 527 nm).
5/6-(3-Aminopropyl)-amido-tetramethylrhodamine (5/6-Aminopropyl-TAMRA)
Non-activated rhodamine dye marker with three-carbon linker and free amino group for derivatization.
6-Carboxy-4′,5′-dichloro-2’7′-dimethoxyfluorescein, single isomer (6-JOE)
Non-activated orange fluorescent dye label
5-Carboxy-4′,5′-dichloro-2’7′-dimethoxyfluorescein, single isomer (5-JOE)
Non-activated orange fluorescent dye label