Description
6-ROX-SE – Long-Wavelength Red Dye for Efficient Amine Labeling
6-Carboxy-X-rhodamine NHS ester (6-ROX-SE) is a single-isomer, amine-reactive rhodamine dye used for covalent labeling of proteins and alkylamino-modified nucleic acids. This long-wavelength fluorophore features bright red emission and high photostability, making it ideal for use in fluorescence-based detection systems such as flow cytometry and confocal laser scanning microscopy.
Performance Highlights
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Amine-reactive NHS ester enables stable covalent labeling
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Excitation/emission maxima: 578 nm / 594 nm
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High extinction coefficient (>95,000 M⁻¹·cm⁻¹)
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Excellent water solubility and red fluorescence signal
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Single-isomer form ensures consistent labeling results
Properties
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CAS Number: 117491-83-5
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Molecular Weight: 631.7
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Empirical Formula: C₃₇H₃₃N₃O₇
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Absorption [λmax]: 578 ± 3 nm
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Emission [λmax]: 594 ± 3 nm
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Extinction Coefficient (ε): >95,000 M⁻¹·cm⁻¹
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Buffer System: PBS, pH 7.4
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Solubility: Acetonitrile, DMF, DMSO
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Appearance: Dark violet solid (powder)
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Storage: −20 °C, dry and protected from light (especially in solution)
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Trade Names: 6-Carboxy-X-rhodamine NHS ester, single isomer; 6-ROX-SE
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Reactive Moiety: NHS ester (forms stable amide linkage with 1° amines)
Application
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Covalent bioconjugation for proteins and peptides
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Labeling of alkylamino-modified oligonucleotides
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Flow cytometry (FACS) and red-channel fluorescence detection
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Ideal for applications requiring long-wavelength fluorescence
