Description
TurboTag 489XL Protein Labeling Kit with Large Stokes Shift for DY-485XL Conjugation
TurboTag 489XL from emp BIOTECH is a high-performance protein labeling and purification kit featuring DY-485XL, a dye with an exceptionally large Stokes shift. Specifically developed for proteins ≥ 25 kDa, including antibodies, the kit uses a highly reactive NHS ester of DY-485XL to ensure efficient and stable conjugation. The resulting protein-dye conjugates exhibit fluorescence excitation at 485 nm and emission at 560 nm, providing enhanced spectral separation and reducing background interference in fluorescence-based assays.
Each kit contains 50 nmol of reactive dye, enabling labeling of up to 15 nmol of protein. TurboTag 489XL supports covalent bond formation through NHS ester chemistry and is compatible with emp BIOTECH’s optimized protocolsfor 1 mg or 100 µg of IgG.
Performance Highlights
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Efficient labeling of proteins ≥ 25 kDa, especially antibodies and enzymes
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Large Stokes shift (excitation/emission at 485/560 nm) minimizes spectral overlap
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NHS ester chemistry ensures covalent, stable protein-dye conjugates
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Label up to 15 nmol protein per 50 nmol dye vial
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Compatible with pre-validated IgG labeling workflows (1 mg / 100 µg)
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Ideal for high-sensitivity fluorescence detection and multiplexing
Properties
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Molecular Weight: 599.67
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Empirical Formula: C₂₉H₃₃N₃O₉S
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Absorption [λmax]: 485 ± 3 nm
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Emission [λmax]: 560 ± 3 nm
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Extinction Coefficient (ε): 50,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of proteins and antibodies for high-contrast detection
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Multiplexed fluorescence imaging and analysis
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Protein quantification and tracking in diagnostic workflows
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Sensitive immunoassays with reduced background noise
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Protein-dye conjugation for large Stokes shift applications
Why Choose TurboTag™ 489XL?
TurboTag 489XL delivers superior labeling performance with the added benefit of a large Stokes shift, improving signal clarity and minimizing spectral overlap. Its optimized chemistry and robust NHS ester reactivity make it a go-to solution for sensitive, high-resolution protein analysis. Ideal for fluorescence-based assays where spectral separation is critical, this kit provides consistent, high-yield conjugation for proteins ≥ 25 kDa—supporting research, diagnostics, and advanced bioassay development with minimal hands-on time.
