Description
TurboTag 331 Protein Labeling Kit for High-Sensitivity MANT Conjugation
TurboTag 331 from emp BIOTECH is a complete protein labeling and purification kit for covalent conjugation of proteins with MANT fluorophore (N-methylanthranilic acid). The kit is ideal for labeling proteins ≥ 25 kDa—especially antibodies—using a highly reactive NHS ester derivative of MANT. The resulting conjugates exhibit distinct fluorescence properties with excitation/emission maxima at 331 nm and 426 nm, offering sensitive detection in UV-excitable fluorescence workflows.
Each vial contains 50 nmol of reactive dye and enables labeling of up to 15 nmol of protein. TurboTag 331 uses NHS ester chemistry to form stable amide linkages under mild conditions. The kit is compatible with emp BIOTECH’s pre-optimized protocols for IgG labeling (1 mg and 100 µg), allowing efficient and reproducible conjugation for antibody detection, molecular tracking, and assay development.
Performance Highlights
-
Fast, efficient fluorescent labeling of proteins ≥ 25 kDa, particularly IgG
-
Strong MANT signal with UV excitation/emission at 331/426 nm
-
NHS ester chemistry ensures stable covalent linkage to primary amines
-
Label up to 15 nmol of protein per 50 nmol dye vial
-
Compatible with protocols for 1 mg or 100 µg of antibody
-
Designed for rapid labeling and downstream purification in one workflow
Properties
-
Molecular Weight: 249.0
-
Empirical Formula: C₁₂H₁₂N₂O₄
-
Absorption [λmax]: 331 ± 3 nm
-
Emission [λmax]: 426 ± 3 nm
-
Extinction Coefficient (ε): 3,100 M⁻¹·cm⁻¹
-
Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
-
Storage: –20 °C, dry, protected from light
-
Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
-
Fluorescent labeling of antibodies and proteins
-
Protein detection in biochemical and immunoassay workflows
-
UV-excitable fluorophore labeling for low-background applications
-
Molecular tracking in fluorescence-based experiments
Why Choose TurboTag™ 331?
TurboTag 331 provides an efficient and highly sensitive method for covalent protein labeling with MANT dye, tailored for UV-excitable fluorescence applications. Its NHS ester-based chemistry enables stable amide formation under mild conditions, making it suitable for delicate biomolecules. Designed for proteins ≥ 25 kDa, including IgG, the kit supports rapid and reproducible workflows with strong blue-range emission. TurboTag 331 ensures a high degree of labeling efficiency, fluorescence integrity, and assay compatibility—ideal for researchers requiring dependable detection in life science and diagnostics.
