Description
5-ROX – High-Purity Red Fluorescent Dye for Biomolecule Labeling
5-Carboxy-X-rhodamine (5-ROX) is a high-purity, non-activated red fluorescent dye with a long-wavelength emission profile. This single-isomer rhodamine derivative is ideal for covalent labeling of proteins and alkylamino-modified nucleic acids, particularly in applications requiring low background and strong signal in the orange-red fluorescence channel. Its high extinction coefficient and stable fluorescence make it suitable for demanding bioconjugation workflows.
Performance Highlights
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Long-wavelength emission ideal for low-background fluorescence detection
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Single-isomer for reproducible and consistent labeling
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Excitation/emission maxima: 576 nm / 601 nm
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Excellent solubility in organic solvents
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Compatible with common labeling protocols for nucleic acids and proteins
Properties
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Molecular Weight: 571.1
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Empirical Formula: C₃₃H₃₁N₂O₅Cl
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Absorption [λmax]: 576 ± 3 nm
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Emission [λmax]: 601 ± 3 nm
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Extinction Coefficient (ε): >85,000 M⁻¹·cm⁻¹
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Buffer System: TE Buffer, pH 8
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Solubility: Methanol, DMF, DMSO
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Appearance: Dark violet solid (powder)
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Storage: −20 °C, under argon; keep dry and protect from light (especially in solution)
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Trade Names: 5-Carboxy-X-rhodamine, single isomer; 5-ROX
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Reactive Moiety: Free carboxylic acid
Application
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Bioconjugation of proteins and alkylamino-modified oligonucleotides
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Ideal for fluorescence detection in the orange-red channel
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Compatible with nucleic acid detection, probe development, and multiplex assays
