Description
5-JOE – Orange Fluorescent Dye for Reliable Biomolecule Labeling
5-Carboxy-4’,5’-dichloro-2’,7’-dimethoxyfluorescein (5-JOE), single isomer, is a non-activated orange fluorescein dye developed for covalent labeling of proteins and alkylamino-modified nucleic acids. Its single-isomer structure ensures consistent spectral performance, making it a dependable reagent for bioconjugation and fluorescence-based applications requiring orange emission.
With an excitation maximum at 521 nm and emission at 549 nm, 5-JOE provides strong signal output and excellent compatibility with TRIS buffer systems. Its high molar extinction coefficient enhances sensitivity in fluorescence detection workflows.
Performance Highlights
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Non-activated orange fluorescein dye
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Single-isomer composition for spectral consistency
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Excitation/emission maxima: 521 nm / 549 nm
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High absorptivity (ε = 87,000 M⁻¹·cm⁻¹)
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Suitable for TRIS buffer at pH 8.0
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Ideal for protein and nucleic acid bioconjugation
Properties
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Molecular Weight: 505.26
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Empirical Formula: C₂₃H₁₄Cl₂O₉
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Absorption [λmax]: 521 nm
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Emission [λmax]: 549 nm
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Extinction Coefficient (ε): 87,000 M⁻¹·cm⁻¹
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Buffer for Fluorescence Readings: TRIS, pH 8.0
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Storage Conditions: Store at room temperature. Keep dry and protect from light, especially in solution.
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Trade Names: 5-Carboxy-4’,5’-dichloro-2’,7’-dimethoxyfluorescein; 5-JOE
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Chemical Name: 4-(4,5-Dichloro-6-hydroxy-2,7-dimethoxy-3-oxo-3H-xanthen-9-yl)-isophthalic acid
Application
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Bioconjugation of proteins and modified nucleic acids
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Fluorescence detection methods requiring orange emission
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Labeling protocols in analytical and life science research
