Description
6-JOE – Orange Fluorescent Dye for Covalent Labeling of Biomolecules
6-Carboxy-4’,5’-dichloro-2’,7’-dimethoxyfluorescein (6-JOE), single isomer, is a non-activated orange fluorescein dye designed for covalent labeling of proteins and alkylamino-modified nucleic acids. This single-isomer derivative offers consistent spectral performance and is ideal for fluorescence-based applications requiring an emission maximum around 549 nm. With excellent absorptivity and a high molar extinction coefficient, 6-JOE delivers reliable results in bioconjugation workflows.
Performance Highlights
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Orange fluorescent dye for protein and oligonucleotide labeling
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Single isomer for consistent spectral performance
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Excitation/emission maxima: 521 nm / 549 nm
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High extinction coefficient (ε = 87,000 M⁻¹·cm⁻¹)
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Compatible with TRIS buffer, pH 8.0
Properties
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Molecular Weight: 505.26
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Empirical Formula: C₂₃H₁₄Cl₂O₉
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Absorption [λmax]: 521 nm
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Emission [λmax]: 549 nm
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Extinction Coefficient (ε): 87,000 M⁻¹·cm⁻¹
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Buffer for Fluorescence Readings: TRIS, pH 8.0
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Storage Conditions: Store at room temperature. Keep dry and protect from light, especially in solution.
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Trade Names: 6-Carboxy-4’,5’-dichloro-2’,7’-dimethoxyfluorescein; 6-JOE
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Chemical Name: 2-(4,5-Dichloro-6-hydroxy-2,7-dimethoxy-3-oxo-3H-xanthen-9-yl)-terephthalic acid
Application
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Bioconjugation of proteins and modified nucleic acids
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Fluorescence-based detection methods
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Labeling protocols requiring orange emission signals
