Description
5/6-Aminopropyl-TAMRA – Red Fluorescent Dye for Stable Biomolecule Conjugation
5/6-(3-Aminopropyl)-amido-tetramethylrhodamine (5/6-Aminopropyl-TAMRA) is a non-activated rhodamine dye with a three-carbon amino-functionalized linker designed for covalent labeling of proteins and alkylamino-modified nucleic acids. This versatile red fluorescent label reacts with activated carboxyl groups or other amine-reactive moieties and provides excellent visibility under standard excitation sources such as mercury-arc and He-Ne lasers.
Although the fluorescence quantum yield of TAMRA conjugates is approximately one-fourth that of fluorescein, its intrinsic brightness and superior photostability often result in stronger signal intensity in microscopy and fluorescence detection systems.
Performance Highlights
-
Non-activated red fluorescent dye with three-carbon amino linker
-
Suitable for labeling proteins and alkylamino-modified nucleic acids
-
Strong excitation at 546 nm (mercury-arc) and 543 nm (green He-Ne laser)
-
Greater photostability compared to fluorescein
-
High visibility in standard fluorescence microscopes
Properties
-
Reactive Moiety: Free amino group
-
Dye Type: Non-activated tetramethylrhodamine
Application
-
Bioconjugation
-
Fluorescence labeling of proteins and oligonucleotides
-
Microscopy using mercury-arc or green He-Ne lasers
-
Fluorescence detection in research workflows
