Description
6-TAMRA-SE – Bright Red Fluorophore for Amine Labeling
6-Carboxytetramethylrhodamine NHS ester (6-TAMRA-SE) is a single-isomer, amine-reactive rhodamine dye ideal for covalent labeling of proteins and alkylamino-modified nucleic acids. Its red fluorescence, photostability, and efficient excitation at commonly used microscopy laser lines make it a versatile tool for confocal imaging and flow cytometry applications. Despite a lower quantum yield compared to fluorescein, 6-TAMRA conjugates often appear brighter due to favorable excitation and intrinsic stability.
Performance Highlights
-
Amine-reactive NHS ester for covalent bioconjugation
-
Excitation/emission maxima: 548 nm / 570 nm
-
High photostability and bright red fluorescence
-
Efficiently excited by mercury-arc (546 nm) and He-Ne (543 nm) lasers
-
Single isomer for consistent labeling
Properties
-
CAS Number: 136977-42-9
-
Molecular Weight: 527.5
-
Empirical Formula: C₂₉H₂₅N₃O₇
-
Absorption [λmax]: 548 ± 3 nm
-
Emission [λmax]: 570 ± 3 nm
-
Extinction Coefficient (ε): >78,000 M⁻¹·cm⁻¹
-
Excitation Source: PBS, pH 7.4
-
Solubility: Acetonitrile, DMF, DMSO
-
Appearance: Violet solid (powder)
-
Storage: −20 °C, dry and protected from light (especially in solution)
-
Trade Names: 6-Carboxytetramethylrhodamine succinimidyl ester, single isomer; 6-TAMRA-SE
-
Reactive Moiety: NHS ester (forms stable amide linkage with 1° amines)
Application
-
Fluorescent labeling of proteins and peptides
-
Bioconjugation for microscopy and FACS
-
Red-channel fluorescence imaging
-
Covalent modification of alkylamino-modified oligonucleotides
