Description
5-TAMRA – High-Purity Red Fluorescent Dye for Biomolecule Labeling
5-Carboxytetramethylrhodamine (5-TAMRA) is a classic, non-activated, single-isomer rhodamine dye widely recognized as a FRET acceptor in fluorescence applications. This high-purity red fluorescent marker is ideal for covalent labeling of proteins and alkylamino-modified nucleic acids. Known for its photostability and efficient excitation at common laser lines (546 nm mercury-arc, 543 nm He-Ne), 5-TAMRA enables robust and consistent fluorescence detection in the red spectral region.
Performance Highlights
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Widely used FRET acceptor for red fluorescence detection
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Single-isomer, non-activated form for precise and reproducible labeling
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Excitation/emission maxima: 548 nm / 570 nm
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Enhanced photostability compared to fluorescein conjugates
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Compatible with standard labeling and microscopy techniques
Properties
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Trade Name: 5-Carboxytetramethylrhodamine
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Reactive Moiety: Free carboxylic acid
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Absorption [λmax]: 548 ± 3 nm
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Emission [λmax]: 570 ± 3 nm
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Appearance (color): Not specified
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Appearance (form): Solid (powder)
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Storage: −20 °C; keep dry and protected from light, especially in solution
Application
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Bioconjugation of proteins and alkylamino-modified oligonucleotides
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Fluorescence resonance energy transfer (FRET) studies
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Confocal microscopy and flow cytometry in the red detection channel
