Description
5-TAMRA-SE – Amine-Reactive Rhodamine Dye for Stable Protein Labeling
5-Carboxytetramethylrhodamine NHS ester (5-TAMRA-SE) is a single-isomer, amine-reactive red fluorescent dye designed for covalent labeling of proteins and alkylamino-modified nucleic acids. Offering high photostability and efficient excitation by common fluorescence microscopy light sources, 5-TAMRA-SE is a preferred choice for applications that demand long-lasting, vivid fluorescence in the orange-red spectral range.
Performance Highlights
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Amine-reactive NHS ester enables robust, stable labeling via amide bond formation
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Excitation/emission maxima: 548 nm / 570 nm
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Readily excited by mercury-arc (546 nm) and He-Ne (543 nm) laser lines
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More photostable than fluorescein dyes
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Single-isomer for reproducible conjugation efficiency
Properties
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CAS Number: 116763-26-9
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Molecular Weight: 527.5
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Empirical Formula: C₂₉H₂₅N₃O₇
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Absorption [λmax]: 548 ± 3 nm
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Emission [λmax]: 570 ± 3 nm
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Extinction Coefficient (ε): >78,000 M⁻¹·cm⁻¹
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Buffer System: PBS, pH 7.4
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Solubility: Acetonitrile, DMF, DMSO
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Appearance: Violet solid (powder)
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Storage: −20 °C, dry and protected from light (especially in solution)
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Trade Names: 5-Carboxytetramethylrhodamine succinimidyl ester, single isomer; 5-TAMRA-SE
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Reactive Moiety: NHS ester (forms stable amide bond with 1° amines)
Application
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Bioconjugation of proteins, peptides, and modified oligonucleotides
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Fluorescence microscopy using 543 nm or 546 nm excitation
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Flow cytometry (FACS) in red-orange channels
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Use in labeling protocols requiring high photostability and long-wavelength fluorophores
