Description
High-purity amine-reactive rhodamine dye for red fluorescence labeling
This amine-reactive red fluorescent dye is a mixed isomer NHS ester of carboxytetramethylrhodamine (TAMRA). It is widely used for covalent labeling of proteins and alkylamino-modified nucleic acids. TAMRA conjugates exhibit a fluorescence quantum yield approximately one-fourth that of fluorescein, yet often appear brighter due to strong excitation by the 546 nm mercury-arc lamp line and enhanced photostability. TAMRA is also efficiently excited by the 543 nm green He-Ne laser, making it well-suited for microscopy and flow cytometry.
Performance Highlights
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Excitation/Emission: 554 nm / 577 nm
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Strong absorbance (ε > 80,000 M⁻¹ cm⁻¹)
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High-purity, mixed isomer format
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Reliable covalent labeling via NHS-ester chemistry
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Compatible with fluorescence imaging and FACS
Properties
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CAS Number: 246256-50-8
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Molecular Weight: 527.5
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Empirical Formula: C₂₉H₂₅N₃O₇
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Absorption [nm]: 554 ± 3
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Emission [nm]: 577 ± 3
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ε [M⁻¹ cm⁻¹]: > 80,000
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Buffer System for Fluorescence Readings: T&S Buffer, pH 9
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Solubility: Acetonitrile, DMF, DMSO
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Appearance (color): Violet
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Appearance (form): Solid powder
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Storage: –20 °C. Keep dry. Protect from light, especially in solution.
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Trade Name: 5/6-Carboxytetramethylrhodamine, succinimidyl ester, mixed isomers; 5/6-TAMRA-SE
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Reactive Moiety: NHS ester, reacts with 1° amines to form a stable amide linkage
Application
Flow cytometry (FACS), bioconjugation of proteins and nucleic acids.
