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High-Capacity Ion Exchange Columns for FPLC Protein Purification

Ion Exchange Chromatography with ZetaSep FF6 Columns

Ion exchange chromatography is a cornerstone in biomolecule purification, offering high resolution and scalability across workflows. emp BIOTECH’s ZetaSep FF6 columns utilize cross-linked 6% agarose resins functionalized with strong and weak anion and cation exchangers. These FPLC-ready columns deliver reliable separation of proteins and other charged biomolecules based on isoelectric point and buffer conditions.

The following formats are available for FPLC purification:

Affinity – For selective binding of antibodies and tagged proteins
Hydrophobic Interaction – Enables gentle purification under high-salt conditions
SMIRF™ – FPLC Screening Kit – For rapid evaluation of ligands and conditions
ZS-1208

Zetasept SP FF6

FPLC column packed with Strong Cation Exchange Agarose Resin. A cross-linked 6 % beaded agarose IEX resin for protein purification.

ZS-1205

ZetaSep DEAE FF6

FPLC column packed with Weak Anion Exchange Agarose Resin. A cross-linked 6 % beaded agarose IEX resin for protein purification.

ZS-1207

ZetaSep CM FF6

FPLC column packed with Weak Cation Exchange Agarose Resin. A cross-linked 6 % beaded agarose IEX resin for protein purification.

ZS-1202

ZetaSep Q FF6

FPLC column packed with Strong Anion Exchange Agarose Resin. A cross-linked 6 % beaded agarose IEX resin for protein purification.