Description
Strong Cation Exchange Column for Reliable Protein Separation
ZetaSep SP FF6 from emp BIOTECH is a 5 mL pre-packed FPLC column filled with Zetarose SP FF6—a 6% cross-linked beaded agarose resin functionalized with sulphopropyl (SP) ligands for strong cation exchange (CEX) chromatography. Designed for high-resolution purification of positively charged biomolecules, the column delivers robust and reproducible separation performance in downstream processing workflows.
The column housing is made of polypropylene with 20 µm polyethylene frits and standard 10/32 fittings for seamless integration into common FPLC systems. ZetaSep SP FF6 supports high flow rates (2–10 mL/min) and pressures up to 3 bar, while its chemically stable matrix tolerates a wide range of aqueous buffers.
Performance Highlights
- Strong cation exchange capacity: 0.14–0.26 mmol H⁺/mL
- Particle range: ≥ 80% between 45–160 µm
- Linear velocity: ≥ 500 cm/h
- Pressure resistance: up to 3 bar
- Broad pH stability: 2–14 (short term), 4–12 (long term)
- Chemically stable in commonly used aqueous buffers
Specifications
- Resin: Zetarose SP FF6
- Column Format: 5 mL FPLC polypropylene housing with 10/32 fittings
- Frits: 20 µm polyethylene
- Storage Buffer: 0.2 M sodium acetate in 20% ethanol
Applications
- Ion exchange chromatography (CEX)
- Protein and enzyme purification
- Scouting and optimization of purification conditions
- Pilot-scale evaluation and small-scale production
Why Choose ZetaSep SP FF6?
ZetaSep SP FF6 offers a convenient and reliable format for strong cation exchange chromatography using Zetarose SP FF6 resin. Pre-packed, pressure-tolerant, and chemically robust, this SMART™-ready FPLC column supports reproducible protein purification with minimal setup—ideal for labs needing high performance in a plug-and-play format.
