Description
Scalable Strong Anion Exchange for High-Yield Protein Purification
ZetaSep Q FF6 from emp BIOTECH is a pre-packed FPLC column containing 6% cross-linked agarose beads functionalized with quaternary ammonium (Q) ligands for strong anion exchange (AEX) chromatography. Designed for high-resolution protein purification and bioprocess development, this column enables efficient separation of negatively charged biomolecules under scalable flow conditions.
Each column is packed with Zetarose Q FF6, offering high flow rates, stable performance across a broad pH range, and excellent chemical resistance. The ~100 µm beads ensure consistent packing, reproducibility, and compatibility with standard FPLC systems, making it ideal for both method development and preparative-scale purification.
Performance Highlights
- Ion exchange capacity: 0.08–0.16 mmol Cl⁻/mL
- Particle diameter: ~100 µm
- Recommended flow rate: 2.0–10.0 mL/min (60–300 cm/h)
- Pressure stable up to 3 bar
- Broad pH stability: 2–14 (short term), 3–12 (long term)
- Compatible with most aqueous buffers (avoid oxidizers and anionic detergents)
Specifications
- Resin: Zetarose Q FF6
- Column Format: Polypropylene, 10/32 fittings, 20 µm frits
- Storage Buffer: 20% ethanol
Applications
- Strong anion exchange (AEX) chromatography
- Protein and enzyme purification
- Separation of negatively charged biomolecules
- Method development and pilot-scale processing
Why Choose ZetaSep Q FF6?
ZetaSep Q FF6 combines strong anion exchange performance with flow stability and robustness. With its pre-packed convenience and reliable packing quality, it offers a seamless path from lab-scale optimization to preparative workflows, making it an essential tool for high-throughput protein purification and biomolecule separation.
