Description
Reliable Weak Anion Exchange for High-Resolution Protein Separation
ZetaSep DEAE FF6 from emp BIOTECH is an FPLC column pre-packed with Zetarose DEAE FF6 resin—a 6% cross-linked beaded agarose functionalized with diethylaminoethyl (DEAE) ligands for weak anion exchange (AEX) chromatography. This column delivers consistent, high-resolution separation of negatively charged biomolecules under mild buffer conditions.
Built on the Zetarose FlashFlow platform, the resin supports high flow rates and excellent mechanical stability, making it ideal for reproducible purification in lab-scale workflows. ZetaSep DEAE FF6 ensures sharp peak profiles and dependable performance across a wide pH range.
Performance Highlights
- Weak anion exchange capacity: 0.08–0.16 mmol Cl⁻/mL resin
- Particle size: ~100 µm average diameter
- Flow rate: 2.0–10.0 mL/min (60–300 cm/h)
- Pressure tolerance: up to 3 bar
- pH stability: 2–14 (short term), 3–12 (long term)
- Tolerant to most aqueous buffers
Specifications
- Matrix: 6% cross-linked beaded agarose
- Ligand: Diethylaminoethyl (DEAE), weak anion exchanger
- Column Volume: 5.0 mL
- Column Housing: Polypropylene with 20 µm frits, 10/32 fittings
- Storage Buffer: 20% ethanol
Applications
- Weak anion exchange chromatography (AEX)
- Protein and enzyme purification
- Separation of negatively charged biomolecules
- Intermediate or polishing steps in downstream processing
Why Choose ZetaSep SP FF6?
ZetaSep DEAE FF6 offers reproducible, gentle purification of anionic proteins and biomolecules. With robust pressure stability and high-resolution performance, it’s the perfect choice for researchers needing scalable, high-throughput separation using an FPLC-compatible weak AEX column.
