Description
Scalable Weak Cation Exchange for Reliable Protein Purification
ZetaSep CM FF6 from emp BIOTECH is a pre-packed FPLC column filled with Zetarose CM FF6, a 6% cross-linked agarose resin functionalized with carboxymethyl (CM) groups for weak cation exchange chromatography (CEX). Designed for high-resolution separation of biomolecules, this column provides reproducible performance in both analytical and preparative workflows.
ZetaSep CM FF6 columns are packed with ~100 µm beads that enable robust flow dynamics while maintaining pressure stability up to 3 bar. The resin offers an ion exchange capacity of 0.08–0.16 mmol H⁺/mL and is chemically stable across a broad pH range. This column is suitable for downstream purification, isoform separation, and intermediate protein capture at lab scale.
Performance Highlights
- Ion exchange capacity: 0.08–0.16 mmol H⁺/mL
- Particle size: ~100 µm beaded agarose
- Pressure-stable up to 3 bar
- Linear flow rate: up to 300 cm/h
- Broad pH stability: 2–14 (short term), 4–12 (long term)
- Stable in aqueous buffers; ethanol-stored
Specifications
- Resin: Zetarose CM FF6
- Column Height: 25 mm
- Bead Size: ~100 µm
- Housing: Polypropylene column, 20 µm frits, 10/32 fittings
Applications
- Ion exchange chromatography (CEX)
- Separation of acidic proteins and isoforms
- Intermediate purification steps
- Lab-scale protein purification
Why Choose ZetaSep CM FF6?
ZetaSep CM FF6 offers efficient weak cation exchange purification in a convenient FPLC-ready format. It combines the selectivity of CM-based chromatography with robust flow properties, making it ideal for reproducible protein purification at small- to mid-scale.
