FPLC Purification Kits for Antibody, IMAC, IEX, and HIC Applications
SMIRF™ - Fast Track Your Protein Purification Protocol
emp BIOTECH’s ZetaSep FPLC columns for hydrophobic interaction chromatography (HIC) offer high-performance purification based on the selective binding of proteins to hydrophobic ligands under high-salt conditions. Featuring phenyl and butyl ligands immobilized on highly cross-linked 6% agarose resins, these columns are optimized for reproducible separation and gentle elution conditions. Whether you require enhanced binding capacity with smaller particle size or a broader interaction range for scalable HIC workflows, ZetaSep columns provide flexibility and robust performance for clarified feed streams.
The following formats are available for FPLC purification:
Ion Exchange – A widely used method based on surface charge
Hydrophobic Interaction – Enables gentle purification under high-salt conditions
SMIRF™ – FPLC Screening Kit – For rapid evaluation of ligands and conditions
SMIRF™ Immobilised Metal Affinity (IMAC) Purification, 4 x 1 mL 1 x 5 mL
Includes 1 mL ZetaSep columns packed with Zetarose Ni-NTA, Zetarose Co-NTA, Zetarose Cu-NTA and Zetarose Zn-NTA solid phases and a 5 mL Zetadex Desalting column.
SMIRF™ Antibody Purification, 2 x 1 mL 1 x 5 mL
Contains a 1 mL base-stable (1 M NaOH) Zetarose Protein A Elevate® column, a 1 mL Zetarose Phenyl hydrophobic interaction (HIC) column and a 5 mL Zetadex Desalting column.
SMIRF™ Ion Exchange (IEX) Purification, 4 x 1 mL 1 x 5 mL
Includes 1 mL ZetaSep columns packed with Zetarose CM, Zetarose DEAE, Zetarose Q and Zetarose SP solid phases and a 5 mL Zetadex Desalting column.