Description
Versatile IMAC Screening with ZetaSep Columns for Polyhistidine-Tagged Proteins
The SMIRF™ Immobilised Metal Affinity (IMAC) Purification Kit from emp BIOTECH provides a robust, flexible solution for screening optimal metal affinity ligands in His-tagged protein purification workflows. This FPLC-compatible kit includes four 1 mL ZetaSep columns packed with Zetarose Ni-NTA, Co-NTA, Cu-NTA, and Zn-NTA resins, along with a 5 mL ZetaSep desalting column filled with Zetadex 25-SF for final buffer exchange or desalting.
All columns are pre-packed and ready-to-use with standard chromatography systems. The agarose-based IMAC resins are derivatized with nitrilotriacetic acid (NTA) and charged with divalent metal ions, enabling selective capture of 6xHis-tagged proteins. The included desalting column allows for rapid post-capture buffer exchange, ensuring compatibility with downstream analysis or formulation.
Performance Highlights
- Includes four 1 mL IMAC columns: Ni²⁺, Co²⁺, Cu²⁺, and Zn²⁺
- Broad-spectrum selectivity for His-tagged proteins
- 5 mL desalting column with cross-linked dextran (Zetadex 25-SF)
- Fast screening of metal affinity options
- Compatible with all standard FPLC systems
- Ready-to-use format, no packing required
Specifications
- Resin: Zetarose Ni-NTA FF6, Co-NTA FF6, Cu-NTA FF6, Zn-NTA FF6; Zetadex 25-SF
- Bead Diameter: 100 µm (agarose-based)
- Column Volume: 1 mL (IMAC), 5 mL (desalting)
- Column Height: 25 mm
- Storage: +2 to +30 °C in 20% ethanol
- pH Stability: Compatible with standard IMAC elution conditions (imidazole, low pH)
Applications
- Screening of optimal IMAC resins for His-tagged proteins
- Purification of recombinant proteins from bacterial or eukaryotic lysates
- Post-capture desalting and buffer exchange
- Optimization of capture conditions for process development
Why Choose the SMIRF™ IMAC Purification Kit?
The SMIRF™ IMAC Purification Kit accelerates discovery and process development by allowing rapid side-by-side comparison of four different IMAC resins. Designed for high-performance His-tag protein capture and downstream desalting, it streamlines method development with reliable performance and universal compatibility with chromatography systems.
