Description
Rapid Desalting and Buffer Exchange with ZetaPrep Desalt 16/100
ZetaPrep Desalt 16/100 is a high-performance prepacked size exclusion chromatography column designed for fast and efficient desalting, buffer exchange, and removal of small molecules from biomolecule samples using liquid chromatography systems. Packed with 20 mL of Zetadex-25 Fine, this column enables reproducible and gentle purification of proteins, enzymes, and nucleic acids larger than 5 kDa with minimal sample dilution or loss.
Ideal for use in FPLC, ÄKTA™, MPLC, or manual setups, ZetaPrep Desalt 16/100 simplifies downstream processing with a recommended flow rate of 2.5–15 mL/min and maximum backpressure of 2 bar. The column body is designed to ensure robust performance, and the matrix exhibits excellent chemical stability across a broad pH range.
Performance Highlights
- Effective MWCO: Approx. 5 kDa for size exclusion of salts, dyes, metal ions, urea, and other low MW impurities
- High Flow Rate Compatibility: Operates efficiently at 2.5–15 mL/min without compromising resolution
- Versatile Use: Compatible with all major chromatography systems and manual workflows
- Chemical Stability: Minimal pH and buffer impact on resolution
- Easy Scale-up: Ideal for preparative and process development needs
Specifications
- Bed Volume: 20 mL
- Recommended Sample Volume: up to 6 mL
- Matrix: Zetadex-25 Fine
- MWCO (approx.): 5 kDa
- Bead Size (hydrated): 20–80 µm
- Flow Rate: 2.5–15 mL/min
- Max. Backpressure: 2 bar
- Column Dimensions: Ø16 mm × 100 mm
- Material: Polypropylene housing
- Inlet/Outlet Fittings: 10–32 (1/16”) female/male
Applications
- Desalting of antibody and protein samples
- Buffer exchange for enzymatic assays or activity studies
- Removal of low MW additives, inhibitors, and reaction by-products
- Sample preparation prior to ion exchange or affinity chromatography
Why Choose ZetaPrep Desalt 16/100?
ZetaPrep Desalt columns combine the speed and simplicity of gravity-based systems with the precision and scalability of FPLC. With emp BIOTECH’s proprietary Zetadex-25 resin, the ZetaPrep platform offers high resolution, low non-specific binding, and excellent lot-to-lot consistency, making it a reliable choice for purification workflows in research and bioprocessing.
