Description
Robust Size Exclusion Resin for Process-Scale Desalting and Buffer Exchange
Zetadex-25 Coarse is a dry, beaded size exclusion resin designed for preparative and process-scale purification of proteins and nucleic acids. Composed of ultrapure cross-linked dextran, this robust gel filtration matrix enables rapid desalting, buffer exchange, and the removal of low molecular weight contaminants.
With bead diameters ranging from 100 to 300 µm, Zetadex-25 Coarse supports excellent flow rates and mechanical stability, making it an ideal choice for gravity flow and large-scale column chromatography. Its molecular weight cut-off (MWCO) of 5 kDa for proteins and 10 base pairs for nucleic acids ensures effective separation of target biomolecules from salts, dyes, and other small molecules.
Performance Highlights
- High-resolution separation based on molecular size
- ≥ 5 kDa exclusion limit for proteins, ≥ 10 bp for nucleic acids
- Coarse bead size (100–300 µm) for optimal flow and column performance
- Minimal dilution of target biomolecules
- Broad pH and buffer compatibility
- Excellent mechanical and chemical stability
Specifications
- Format: Dry powder
- Bead Diameter (dry): 100–300 µm
- Matrix Composition: Cross-linked dextran
- Molecular Weight Cut-Off (MWCO): 5 kDa (proteins), 10 bp (nucleic acids)
- Fractionation Range: 1–5 kDa for globular proteins
- Water Regain: 2.15–2.25 mL/g
- Swelling Volume: 4–6 mL/g (dependent on buffer conditions)
- Autoclavable: 121 °C for 30 minutes at pH 7
Applications
- Desalting of proteins and nucleic acids
- Buffer exchange before downstream processing
- Process chromatography using gravity-flow or low-pressure systems
Why Choose Zetadex-25 Coarse?
Zetadex-25 Coarse delivers consistent, high-performance desalting and buffer exchange for large biomolecules. With robust physicochemical properties and broad solvent compatibility, it is ideally suited for preparative-scale workflows in bioprocessing, molecular biology, and nucleic acid research.
