Description
Reliable Size Exclusion for Mid-Scale Protein and Nucleic Acid Purification
Zetadex-25 Fine from emp BIOTECH is a dry, beaded composite gel filtration matrix engineered for desalting and buffer exchange of proteins (≥5 kD) and nucleic acids (≥10 bp). Composed of cross-linked dextran, it offers high resolution, excellent selectivity, and broad buffer compatibility—ideal for size-based separation in research and manufacturing workflows.
Zetadex-25 Fine is optimized for aqueous systems and supports efficient separation under a wide pH range. Its stable spherical beads ensure reproducibility and consistent flow properties, even during extended purification runs. This matrix is suitable for gravity-flow, low-pressure, and FPLC systems.
Performance Highlights
- Size exclusion cut-off: ~5 kD (proteins), ~10 bp (nucleic acids)
- Dry bead diameter: 20–80 µm
- Wet bead diameter: 35–140 µm
- ≥ 80% of beads within the specified size range
- Broad chemical compatibility including 0.2 M NaOH, 8 M urea, 6 M guanidine HCl
- pH stability: 2.0–13.0
- Autoclavable: 121 °C at pH 7 for 30 minutes
- Pressure-stable; usable up to 3 bar under Darcy’s Law flow conditions
Specifications
- Matrix: Cross-linked spherical dextran composite
- Bead Size (Dry): 20–80 µm
- Bead Size (Wet): 35–140 µm
- Size exclusion limit: 5 kDa (proteins) / 10 bp (nucleic acids)
- Water Regain: 2.15–2.25 mL/g
- Swelling Factor: 4–6 mL/g
- Appearance: White dry powder
- Autoclavable: Yes (121 °C, pH 7, 30 min)
Applications
- Desalting of protein and nucleic acid samples
- Buffer exchange prior to ion exchange or affinity chromatography
- Size-based separation of biomolecules in analytical and preparative workflows
- Mid-scale purification in life science and diagnostic manufacturing
Why Choose Zetadex-25 Fine?
Zetadex-25 Fine is designed for precise desalting and size exclusion in aqueous systems. Its consistent bead geometry, wide chemical resistance, and strong pressure and pH tolerance make it an ideal choice for reliable biomolecule purification at scale. Whether you’re preparing samples for downstream chromatography or removing small molecules, this matrix ensures reproducible results.
