Description
High-capacity desalting and purification for nucleic acids, proteins, and nanoparticles
The CentriPure 100 – Z50M from emp BIOTECH is a ready-to-use, gravity-driven gel filtration column designed for high-efficiency desalting and buffer exchange of larger biomolecules. Precision-packed with Zetadex-50, a cross-linked beaded dextran resin, this column delivers rapid and selective removal of small molecules such as salts, dyes, biotin, ammonia, and haptens from proteins >25 kDa, nucleic acids longer than 20 bp, and nanoparticles >4 nm.
With a 10 mL sample capacity and a fast protocol completed in under 15 minutes, CentriPure 100 – Z50M enables highly reproducible purification results with >99.99% small molecule removal, >85% recovery, and ~1.5-fold dilution. This format is ideal for preparative workflows in molecular biology, proteomics, nanoparticle research, and diagnostic assay development.
Performance Highlights
- 99.99% removal of low molecular weight impurities
- 85% recovery of proteins and nucleic acids
- ~1.5-fold sample dilution
- <15-minute gravity-flow purification
- Alternative to dialysis and TFF
- Format: Individual hydrated gel filtration column
- Workflow: No centrifugation or vacuum required
Specifications
- Resin: Zetadex-50 (50–150 µm particle size)
- Sample volume: 10 mL
- Output volume: ~14 mL
Applications
- DNA/RNA desalting
- Protein purification
- Buffer exchange
- Oligonucleotide cleanup
Why Choose CentriPure 100 – Z50M?
Optimized for medium- to large-scale biomolecule preparations, CentriPure 100 – Z50M is a powerful alternative to time-consuming dialysis and complex ultrafiltration systems. With its automation-free, gravity-based design and endotoxin-free optional variant, it is the solution of choice for reliable and scalable purification in both R&D and production settings.
