Description
Robust Size Exclusion for Mid-Range Biomolecule Separation
Zetadex-50 Medium from emp BIOTECH is a dry, cross-linked composite gel filtration matrix engineered for efficient separation of proteins (~25 kD) and nucleic acids (~20 bp). This beaded dextran-based size exclusion resin offers a dry particle range of 50–150 µm and swells to 100–300 µm upon hydration. It provides high resolution, excellent physical stability, and broad chemical compatibility, making it ideal for process purification workflows.
Zetadex-50 Medium separates molecules based on size, ensuring minimal dilution and buffer interference. Its robustness in a wide pH range and compatibility with aggressive buffers such as NaOH, guanidine HCl, and alcohols supports both routine and demanding purification protocols.
Performance Highlights
- Size exclusion cutoff: ~25 kD (proteins) and ~20 bp (nucleic acids)
- Bead size: 50–150 µm (dry); 100–300 µm (hydrated)
- Swelling capacity: 9–11 mL/g
- Water regain: 4.8–5.2 mL/g
- Autoclavable: 121 °C, pH 7, for 30 minutes
- pH stability: 2.0–10.0
- Chemical compatibility: Stable in 0.2 M NaOH, 6 M guanidine HCl, 30% acetonitrile, and more
- Maximum pressure: up to 3 bar
Specifications
- Matrix: Cross-linked dextran composite
- Size exclusion limit: 25 kDa (proteins) / 20 bp (nucleic acids)
- Particle size: 50–150 µm (dry)
- Swelling volume: 9–11 mL/g
- Appearance: White powder
Applications
- Desalting and buffer exchange
- Separation of mid-sized biomolecules
- Size exclusion purification of proteins and nucleic acids
- Lab-scale and preparative chromatography
Why Choose Zetadex-50 Medium?
Zetadex-50 Medium offers robust, reproducible separations for a wide range of biomolecules. Its uniform particle size and compatibility with strong solvents and pH extremes make it a versatile choice for researchers and process developers requiring consistent performance with minimal sample loss or interference.
