Description
Fast and Reliable Desalting for Small-Volume Preparations
ZetaSep Desalt is a prepacked FPLC column with a 1 mL bed volume designed for rapid and efficient desalting, buffer exchange, and removal of low molecular weight contaminants. Engineered for use with liquid chromatography systems, this column enables reproducible separation of larger biomolecules—such as antibodies, enzymes, and nucleic acids—from unwanted small molecules using size-exclusion principles.
Ideal for small-scale workflows, ZS-0102 allows simple and effective purification using a syringe, peristaltic pump, or standard chromatography system. It contains Zetadex-25 Superfine, a composite beaded gel matrix manufactured by emp BIOTECH in Berlin-Buch. The resin supports high flow rates, excellent chemical stability, and consistent resolution across a wide pH range.
Performance Highlights
- Pre-packed 1 mL column for fast processing of sample volumes between 0.02–0.3 mL
- Fractionation range: ~1–5 kDa (globular proteins)
- Size exclusion cut-off: ~5 kDa
- Compatible with MPLC, FPLC™, ÄKTAdesign™, and manual systems
- Matrix: Zetadex-25 Superfine for high resolution and reproducibility
- Flow rate: 0.5–3 mL/min
- Max. backpressure: 3 bar
Specifications
- Column volume: 1 mL
- Sample volume range: 20–300 µL
- Bead diameter (hydrated): 20–85 µm
- Matrix material: Zetadex-25 Superfine (dextran composite)
- Column body: Polypropylene
- Ports: 10–32 (inlet female, outlet male)
- Storage buffer: 20% ethanol
- Storage temperature: +2 °C to +30 °C (Do not freeze)
- pH stability: 2–13
Applications
- Desalting
- Buffer exchange
- Removal of low molecular weight compounds
- Reaction termination
- Preparation of protein eluates for downstream analysis
Why Choose ZetaSep Desalt 1 mL?
ZS-0102 offers exceptional convenience and performance for small-volume desalting and buffer exchange tasks. The consistent matrix and precision-packed format ensure reliable separation of high-value proteins and biomolecules from salts and small molecule contaminants. Designed for reproducibility and ease-of-use, this column integrates seamlessly into routine workflows across research and process development labs.
