Description
Reliable Weak Anion Exchange Resin for Protein and Nucleic Acid Purification
Zetadex-50 DEAE from emp BIOTECH is a dry, beaded composite gel filtration resin, functionalized with diethylaminoethyl (DEAE) groups for weak anion exchange (AEX) chromatography. Developed from cross-linked dextran (Zetadex-50), this matrix offers high capacity, chemical robustness, and predictable performance in the separation of negatively charged biomolecules such as nucleic acids and proteins.
This versatile resin is suitable for gravity flow, centrifugation, and chromatography system formats, and is particularly useful for gentle purification protocols, even in the presence of denaturants such as urea or guanidine hydrochloride.
Performance Highlights
- Ion exchange type: Weak anion exchanger (DEAE)
- Total ionic capacity: ~3 mmol/g dry material
- Binding capacity: ~100 mg BSA/mL gel bed
- Bead size (dry): 40–120 µm (> 80%)
- Water regain: 12 ± 2 mL/g (in 150 mM NaCl)
- Swelling: 20–25 mL/g (in 150 mM NaCl)
- pH working range: 2–9
- pH stability: 2–12
- Chemical stability: Stable in all commonly used aqueous buffers, incl. 8 M urea and 6 M guanidine hydrochloride
- Physical stability: Minor volume changes with ionic strength or pH
Specifications
- Matrix: Cross-linked dextran composite
- Ligand: Diethylaminoethyl (DEAE)
- Form: Dry powder
- Bead diameter (dry): 40–120 µm
- Hydration volume: 1 g dry resin yields ~20–25 mL gel
Applications
- Ion Exchange Chromatography (IEX)
- Protein and nucleic acid purification
- Process development and buffer optimization
- Scouting of weakly anionic biomolecules
- Use in aqueous or denaturing purification buffers
Why Choose Zetadex-50 DEAE?
Zetadex-50 DEAE delivers predictable weak anion exchange performance, ideal for lab-scale and process-scale applications alike. With excellent capacity, pH resilience, and compatibility with denaturing buffers, it’s the preferred solution for reproducible purification of proteins, oligonucleotides, and other anionic biomolecules.
