Description
High-Capacity Weak Cation Exchange Chromatography
Zetadex-25 CM is a high-performance weak cation exchange chromatography resin developed by emp BIOTECH. Based on a cross-linked dextran composite matrix, the resin is derivatized with carboxymethyl (CM) groups to enable the selective binding and purification of proteins and nucleic acids carrying a net positive charge under specific buffer conditions.
This resin combines high ionic exchange capacity with chemical robustness, delivering reproducible performance in protein purification workflows. Zetadex-25 CM is ideal for desalting, fractionation, and polishing steps in downstream processing.
Performance Highlights
- Strong binding power: 4–5 meq/g ionic capacity (dry basis)
- Rapid equilibration & efficient recovery: Fast flow rates without bead deformation
- Customizable swelling properties: Gel volume of 5–7 mL per gram of dry powder
- Easy integration: Compatible with standard chromatography equipment and formats
Specifications
- Matrix composition: Cross-linked spherical dextran composite
- Functional ligand: Carboxymethyl (CM)
- Bead size (dry): 50–150 µm
- Bead size (hydrated): 90–280 µm
- Ionic capacity: 4–5 mmol/g (dry)
- Water regain: 3–5 mL/g
- Swelling: 6–8 mL/g
- pH working range: 6–10
- pH stability range: 2–13
- Autoclavable: 121 °C, 0.1 M NaCl, 30 minutes
Applications
- Ion Exchange Chromatography (IEX) for purification of positively charged biomolecules
- Desalting and buffer exchange
- Intermediate purification steps in protein downstream processing
Why Choose Zetadex-25 CM?
Zetadex-25 CM offers an ideal balance of selectivity, stability, and flow performance for weak cation exchange applications. Its optimized bead size and narrow distribution ensure consistent results and high-resolution separations, even at larger scales. The resin’s chemical robustness and compatibility with a wide pH range make it suitable for varied downstream conditions, while its efficient swelling properties and easy handling support seamless integration into purification workflows.
