Description
Rapid desalting and buffer exchange of nucleic acids, proteins, and nanoparticles in low-volume formats
he CentriPure 2 – Z50M from emp BIOTECH is a high-performance gel filtration column designed for the fast and efficient removal of small molecules such as salts, dyes, ammonia, biotin, and haptens from biomolecules. Utilizing Zetadex-50, a cross-linked dextran-based size-exclusion resin, this column enables gravity-based purification and buffer exchange of proteins (>25 kDa), nucleic acids (>20 bp), and spheroidal nanoparticles (>4 nm) in just one step.
With a working sample volume range of 150–300 µL, CentriPure 2 is ideal for labs processing smaller sample batches without sacrificing purification quality. Each column delivers >99.99% removal of low-molecular-weight contaminants and >85% recovery of target biomolecules with ~1.5–2-fold dilution, offering a reliable alternative to dialysis and ultrafiltration.
Performance Highlights
- 99.99% removal of small molecules
- 85% recovery of nucleic acids and proteins
- Sample dilution: ~1.5–2-fold
- Processing time: <15 minutes
- Gravity-driven—no centrifugation required
Specifications
- Resin: Zetadex-50 (50–150 µm particle size)
- Sample volume: 150–300 µL
- Elution volume: Up to 500 µL depending on sample/chase volume
- Flow: Gravity-based purification
- Format: Hydrated, pre-filled column
Applications
- DNA/RNA desalting
- Protein purification
- Buffer exchange
- Nanoparticle cleanup
Why Choose CentriPure 2 – Z50M?
The CentriPure 2 – Z50M is optimized for rapid, reproducible purification in low-volume workflows. Its gravity-flow format enables efficient sample handling without specialized equipment, making it ideal for labs looking for speed, reliability, and precision in small-scale biomolecule processing.
