Description
High-Capacity Weak Cation Exchange Matrix for Gentle Protein Purification
Zetadex-25 CM, hydrated is a ready-to-use, weak cation exchange resin based on cross-linked dextran. This pre-hydrated matrix is derivatized with carboxymethyl (CM) functional groups and is optimized for the purification of positively charged biomolecules under mild conditions. With its high ionic capacity and robust gel structure, it supports efficient protein and nucleic acid separations where biological activity must be preserved.
The resin is hydrated in a stabilized aqueous solution for immediate use in process-scale or research chromatography workflows. Its particle size and porosity make it especially suitable for fast separations and gentle elution of delicate biomolecules.
Performance Highlights
- Weak cation exchange resin pre-hydrated for direct use
- Based on Zetadex-25 Medium matrix with high chemical stability
- Carboxymethyl derivatization for mild binding and elution conditions
- Suitable for purification of proteins, enzymes, and nucleic acids
- Consistent performance in gravity, column, or batch purification workflows
Specifications
- Matrix Base: Cross-linked dextran (Zetadex-25 Medium)
- Functional Group: Carboxymethyl (CM)
- Exchange Type: Weak Cation Exchange (CEX)
- Bead Diameter (dry): ~50–150 µm
- Ionic Capacity: 4.0–5.0 mmol/g
- Storage Buffer: Stabilized aqueous solution (typically with sodium azide or ProClin)
- pH Stability Range: 4–9
- Format: Pre-hydrated bulk resin
Applications
- Ion exchange chromatography (CEX)
- Capture and polishing of positively charged proteins
- Protein and enzyme purification from crude or clarified samples
- Scalable workflows from lab to process chromatography
Why Choose Zetadex-25 CM, Hydrated?
Zetadex-25 CM, hydrated delivers all the advantages of a high-performance weak cation exchanger in a convenient ready-to-use format. Its carboxymethyl functionalization ensures selective yet gentle interaction with target molecules—ideal for applications where maintaining structural and functional integrity is critical. With excellent resolution and reproducibility across a wide pH range, this resin is a versatile choice for chromatography workflows that demand high yield and reliability.
