Description
Robust Gel Filtration Matrix for Large-Scale Desalting and Buffer Exchange
Zetadex-50 Coarse from emp BIOTECH is a dry, composite gel filtration resin developed for efficient size-based separation of proteins and nucleic acids in process-scale purification workflows. With a bead diameter of 100–300 µm (dry), this cross-linked dextran-based resin offers exceptional resolution, chemical stability, and flow performance under gravity or low-pressure conditions.
Designed for applications that demand scalability and reliability, Zetadex-50 Coarse enables rapid desalting, buffer exchange, and removal of low-molecular-weight contaminants without diluting the purified biomolecules.
Performance Highlights
- Size Exclusion Cut-off (MWCO): ~25 kDa (proteins), ~20 bp (nucleic acids)
- Bead diameter (dry): 100–300 µm
- Matrix: Cross-linked dextran composite
- Chemical stability: Compatible with most aqueous buffers
- pH range: Broad stability across physiological conditions
- Flow properties: Optimized for gravity and large-volume process setups
- Application: Desalting, buffer exchange, and purification of proteins and nucleic acids in large-scale processes
Specifications
- Matrix: Zetadex-50 Coarse
- Dry bead size: 100–300 µm
- MWCO: ~25 kDa for proteins / ~20 bp for nucleic acids
- Form: Dry powder
- Storage: Cool, dry place (moisture-sensitive)
- Composition: Cross-linked spherical dextran (CAS 9004-54-0)
Applications
- Process chromatography for desalting and buffer exchange
- Purification of antibodies, enzymes, and nucleic acids
- Removal of salts, dyes, and small molecules from large biomolecules
Why Choose Zetadex-50 Coarse?
Zetadex-50 Coarse provides a reliable and scalable solution for downstream purification where high throughput, reproducibility, and resolution are essential. The resin’s large particle size ensures optimal flow for large-volume processes, making it an ideal choice for pilot and production-scale applications requiring consistent desalting or buffer exchange without compromising product integrity.
