Hydrophobic Interaction Chromatography for Robust Protein Separation
Hydrophobic Interaction
emp BIOTECH’s ZetaSep FPLC columns for hydrophobic interaction chromatography (HIC) offer high-performance purification based on the selective binding of proteins to hydrophobic ligands under high-salt conditions. Featuring phenyl and butyl ligands immobilized on highly cross-linked 6% agarose resins, these columns are optimized for reproducible separation and gentle elution conditions. Whether you require enhanced binding capacity with smaller particle size or a broader interaction range for scalable HIC workflows, ZetaSep columns provide flexibility and robust performance for clarified feed streams.
The following formats are available for FPLC purification:
Ion Exchange – A widely used method based on surface charge
Hydrophobic Interaction – Enables gentle purification under high-salt conditions
SMIRF™ – FPLC Screening Kit – For rapid evaluation of ligands and conditions
ZetaSep Phenyl FF6
FPLC column packed with HIC resin, derivatized with phenyl ligand. A cross-linked 6% beaded agarose resin for hydrophobic interaction chromatography purification.
ZetaSep Phenyl HC
FPLC column packed with high capacity HIC resin, derivatized with phenyl ligand. A small-sized cross-linked beaded agarose resin for hydrophobic interaction chromatography purification.
ZetaSep Butyl FF6
FPLC column packed with HIC resin, derivatized with butyl ligand. A cross-linked 6% beaded agarose resin for hydrophobic interaction chromatography purification.