Description
Robust Hydrophobic Interaction Chromatography in FPLC Format
ZetaSep Butyl FF6 from emp BIOTECH is a ready-to-use FPLC column pre-packed with Zetarose Butyl FF6—a 6% cross-linked beaded agarose resin functionalized with butyl (C4H9) ligands. Designed for hydrophobic interaction chromatography (HIC), this column enables salt-dependent purification of proteins, including antibodies and enzymes, under native conditions.
The 100 µm beads ensure optimal flow dynamics, chemical stability, and reproducible retention behavior across a wide range of biomolecules. ZetaSep Butyl FF6 is ideally suited for gradient or stepwise elution in mid-scale HIC workflows.
Performance Highlights
- Moderate hydrophobicity for controlled HIC separations
- 6% cross-linked agarose for mechanical and chemical stability
- Average particle size: 100 µm
- Column height: 25 mm; volume: 5 mL
- Compatible with salt gradients (e.g., ammonium sulfate)
- Pre-packed and ready to use on standard FPLC systems
Specifications
- Matrix: 6% highly cross-linked beaded agarose
- Ligand: Butyl (C4H9)Average Bead Diameter: 100 µm
- Column Volume: 5 mL
- Column Height: 25 mm
- Storage Buffer: 20% ethanol
Applications
- Hydrophobic interaction chromatography (HIC)
- Purification of proteins, antibodies, and fusion constructs
- Intermediate or polishing steps in downstream workflows
- Separation based on surface hydrophobicity
Why Choose ZetaSep Butyl FF6?
ZetaSep Butyl FF6 combines reliable performance and salt-regulated selectivity in a pre-packed column format. Its moderate hydrophobic interaction strength makes it suitable for challenging protein separations where mild, non-denaturing conditions and high resolution are required.
