Description
Reliable Hydrophobic Interaction Chromatography for Protein Purification
ZetaSep Phenyl FF6 from emp BIOTECH is a pre-packed FPLC column filled with Zetarose Phenyl FF6 resin—6% cross-linked beaded agarose functionalized with aromatic phenyl ligands. Designed for hydrophobic interaction chromatography (HIC), this column enables gentle, salt-dependent purification of proteins, enzymes, and other biomolecules based on their surface hydrophobicity.
The column uses phenyl ligands to provide moderate hydrophobic interactions that can be fine-tuned via salt gradients, making it ideal for preserving protein activity during purification. Its 100 µm beads are engineered for efficient flow, high resolution, and compatibility with standard FPLC systems.
Performance Highlights
- Salt-dependent separation based on hydrophobicity
- 100 µm agarose beads ensure optimal resolution
- Suitable for mild, non-denaturing purification conditions
- Compatible with ammonium sulfate, NaCl, or Na₂SO₄ buffers
- Column height: 25 mm (5 mL bed volume)
- Fast equilibration and elution steps
Specifications
- Matrix: 6% highly cross-linked beaded agarose
- Ligand: Phenyl (–C₆H₅), aromatic hydrophobic ligand
- Ligand Density: ~20 µmol/mL
- Bead Diameter: Average 100 µm
- Column Height: 25 mm
- Storage Buffer: 0.2 M sodium acetate in 20% ethanol
- pH Stability: 3–10
- Format: Pre-packed FPLC column
Applications
- Hydrophobic interaction chromatography (HIC)
- Protein and enzyme purification
- Intermediate or polishing steps in DSP
- Separation of biomolecules based on surface hydrophobicity
Why Choose ZetaSep Phenyl FF6?
ZetaSep Phenyl FF6 enables scalable, mild purification of biomolecules with minimal impact on structure and function. Its phenyl-functionalized agarose matrix provides selective hydrophobic interaction in high-salt buffers and ensures reliable performance in downstream processing workflows.
