Description
High-Capacity Phenyl Resin for Precision HIC Purification
ZetaSep Phenyl HC (ZS-1504) is an FPLC column packed with Zetarose Phenyl HC—a 6% cross-linked agarose resin featuring 30 µm beads functionalized with aromatic phenyl (C₆H₅) ligands. Its small particle size and high ligand density (40 µmol/mL) deliver elevated binding capacity and resolution, making it ideal for demanding hydrophobic interaction chromatography (HIC) applications.
The phenyl ligand enables salt-dependent interactions with hydrophobic regions of proteins and biomolecules. This allows effective separation and polishing under gradient conditions. ZetaSep Phenyl HC offers reliable scale-up and reproducibility across purification workflows.
Performance Highlights
- Functional group: Phenyl (C₆H₅)
- Ligand density: 40 µmol/mL resin
- Bead size: 30 µm
- Max. flow rate: > 100 cm/h (tested in 32 mm column ID)
- Matrix: 6% cross-linked agarose
- Format: 25 mm column height, 5 mL FPLC column
Specifications
- Resin: Zetarose Phenyl HC
- Average Bead Diameter: 30 µm
- Application: Hydrophobic Interaction Chromatography (HIC)
- Storage: 20% ethanol, 2–30 °C
- Chemical Stability: Compatible with standard aqueous buffers
Applications
- Separation of hydrophobic proteins and enzymes
- Intermediate and polishing steps in protein purification
- Scouting and optimization of HIC gradients in FPLC workflows
- Suitable for clarified feed streams
Why Choose ZetaSep Phenyl HC?
ZetaSep Phenyl HC offers the high-resolution performance needed for advanced HIC applications. Its small bead size improves surface area contact, while the high ligand density supports high dynamic binding capacities. Whether for protein polishing, aggregate removal, or gradient optimization, this resin supports robust purification under variable salt conditions with consistent performance.
