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High-Performance Size Exclusion Chromatography for Rapid Desalting and Buffer Exchange

FPLC Purification, Size Exclusion – ZetaSep and ZetaPrep Columns for Rapid Removal of Salts and Small Molecules

emp BIOTECH’s ZetaSep and ZetaPrep columns are engineered for fast, reproducible size exclusion chromatography using FPLC and other LC systems. Packed with Zetadex-25 Superfine resin, they efficiently separate biomolecules from low-molecular-weight contaminants like salts, dyes, and solvents. These columns are ideal for proteins, oligonucleotides, and other macromolecules larger than 5 kDa and are available in multiple formats to suit different flow rates and sample volumes.

Available formats:

 

Product Name

Product Number

Bed Volume

Recommended Sample Volume

Flow Rate Range

ZetaSep Desalt 1 mL

ZS-0102

1 mL

0.02–0.3 mL

0.5–3 mL/min

ZetaSep Desalt 5 mL

ZS-0101

5 mL

0.1–1.5 mL

1–15 mL/min

ZetaPrep Desalt 16/100

ZP-0101

20 mL

up to 6 mL

2.5–15 mL/min

ZS-0102

ZetaSep Desalt, 1 mL bed volume

For desalting, removal of small molecules, and buffer exchange using Liquid Chromatography systems. 1 mL bed volume, sample volume 0.02 to 0.3 mL. Flow rate 0.5 to 3 mL/min. Max. Backpressure: 3 bar

ZS-0101

ZetaSep Desalt

For desalting, removal of small molecules, and buffer exchange using Liquid Chromatography systems. 5 mL bed volume, sample volume 0.1 to 1.5 mL. Flow rate 1 to 15 mL/min. Max. Backpressure: 3 bar

ZP-0101

ZetaPrep Desalt 16/100

For desalting, removal of small molecules, and buffer exchange using Liquid Chromatography systems. 20 mL bed volume, flow rate 2.5 to 15 mL/min. Max. Backpressure of resin: 2 bar, recommended sample volume up to 6 mL