Fluorescent Dyes for Bioconjugation and Detection Applications
Fluorescent Dyes: Excitation 350–500 nm
Green-fluorescent dyes for bioconjugation and detection
This section includes high-purity fluorescein-based dyes such as FAM, FITC, and maleimide derivatives, with excitation between 350 and 500 nm. Available in thiol- and amine-reactive forms, they’re ideal for protein and oligo labeling in microscopy, flow cytometry, and biosensing.
For other spectral options, see also:
Excitation 500–600 nm – Orange and red-fluorescent rhodamines and TAMRA-based labels
Excitation 600–700 nm – Far-red fluorescent dyes such as ROX and X-rhodamine
Excitation 350–500 nm
5/6-Carboxyfluorescein NHS ester, mixed isomers (5/6-FAM-SE)
Amine-reactive fluorescein dye marker for labeling of biomolecules (Abs: 496 nm, Em: 517 nm).
5/6-Carboxy-Erythrosin B NHS ester, mixed isomers (EryB-SE)
Amine-reactive singlet-oxygen generator and dye marker labeling of biomolecules (Abs: 527 nm).
Fluorescein-5/6-isothiocyanate (5/6-FITC)
Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 494 nm, Em: 518 nm).
6-Carboxyfluorescein NHS ester, single isomer (6-FAM-SE)
Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 497 nm, Em: 519 nm).
5-Carboxyfluorescein NHS ester, single isomer (5-FAM-SE)
Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 495 nm, Em: 519 nm).
5-Iodoacetamidofluorescein (5-IAAF)
Thiol-reactive fluorescein derivative for fluorescence derivatization, especially for labeling of cysteines and thiol-modified oligos.
Fluorescein-5-maleimide (5-F-MI)
Thiol-reactive fluorescein derivative for fluorescence derivatization, especially for labeling of cysteines and thiol-modified oligos.
5-Carboxyfluorescein, single isomer (5-FAM) (99+%, high purity)
High purity, non-activated fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 493 nm, Em: 517 nm).
5/6-Carboxyfluorescein, mixed isomers (5/6-FAM) (99+%)
High purity, non-activated fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 496 nm, Em: 517 nm).
6-Carboxyfluorescein, single isomer (6-FAM) (99+%, high purity)
High purity, non-activated fluorescein dye marker for fluorescence lableing, especially for labeling of biomolecules (Abs: 493 nm, Em: 514 nm).
6-(3-Aminopropyl)-amidofluorescein, single isomer (6-Aminopropyl-FAM)
Non-activated yellow fluorescent dye label, with three-carbon linker and free amino group for derivatization.