Description
6-Aminopropyl-FAM – Flexible Fluorescein Label with Amino Linker for Biomolecule Conjugation
6-(3-Aminopropyl)-amidofluorescein (6-Aminopropyl-FAM), single isomer, is a non-activated yellow fluorescent dye featuring a three-carbon amino linker for flexible covalent attachment. Its excitation and emission spectra (496/517 nm) align perfectly with 488 nm argon-ion lasers, making it an ideal choice for fluorescence labeling in microscopy, flow cytometry, and bioconjugation workflows. With excellent water solubility, high absorptivity, and strong fluorescence yield, it provides consistent, high-signal labeling of proteins and modified oligonucleotides.
Performance Highlights
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Single isomer fluorescein dye with amino linker for derivatization
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Excitation/emission maxima: 496 nm / 517 nm
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igh molar absorptivity (>70,000 M⁻¹·cm⁻¹) and quantum yield
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Compatible with 488 nm laser systems
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Excellent solubility and labeling stability in aqueous buffers
Properties
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Molecular Weight: 546.45
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Empirical Formula: C₂₆H₂₁F₃N₂O₈
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Absorption [λmax]: 496 ± 3 nm
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Emission [λmax]: 517 ± 3 nm
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Extinction Coefficient (ε): >70,000 M⁻¹·cm⁻¹
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Reactive Moiety: Free amino group
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Solubility: Water, methanol, DMF, DMSO
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Buffer for Fluorescence Readings: T&S Buffer, pH 9
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Appearance: Yellow solid (powder)
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Storage: Store at room temperature. Keep dry and protect from light, especially in solution.
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Trade Names: 6-(3-Aminopropyl)-amidofluorescein TFA salt, single isomer
Application
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Protein and nucleic acid labeling
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Fluorescence-based detection systems
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Confocal laser scanning microscopy
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Flow cytometry (FACS)
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Bioconjugation of biomolecules
