Description
5/6-Carboxy-Erythrosin B NHS Ester (EryB-SE) – Amine-Reactive Red Dye and Singlet Oxygen Generator
AF-0114 5/6-Carboxy-Erythrosin B NHS ester (EryB-SE) is a red, amine-reactive labeling reagent designed for the covalent conjugation of biomolecules such as proteins and alkylamino-modified nucleic acids. As a derivative of erythrosin B, it serves as both a fluorescent dye and an efficient generator of singlet oxygen, making it suitable for redox-based and photodynamic applications.
Performance Highlights
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NHS ester enables stable covalent linkage to 1° amines
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Strong absorption maximum at 527 ± 3 nm
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Functions as both a red fluorescent dye and singlet oxygen generator
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Ideal for use in flow cytometry (FACS) and related fluorescence detection methods
Properties
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Trade Name: 5/6-Carboxy-Erythrosin B NHS ester, mixed isomers
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Reactive Moiety: NHS ester, reacts with 1° amines to form a stable amide linkage
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Molecular Weight: 976.97
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Empirical Formula: C₂₅H₁₁NO₉I₄
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Absorption [nm]: 527 ± 3
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Appearance (color): Red
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Appearance (form): Solid (powder)
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CAS Number: None known
Application
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Fluorescent labeling in flow cytometry (FACS)
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Bioconjugation for singlet oxygen generation
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Dye-based detection in biochemical assays
