Description
6-FAM – High-Purity Single Isomer Fluorescein for Precision Biomolecule Labeling
6-Carboxyfluorescein (6-FAM), single isomer, is a non-activated fluorescein dye of 99+% purity, ideal for covalent labeling of proteins and alkylamino-modified nucleic acids. Its excitation profile aligns with the 488 nm argon-ion laser, making it exceptionally well-suited for confocal microscopy, flow cytometry, and fluorescence-based detection methods. With high absorptivity, excellent fluorescence quantum yield, and strong water solubility, 6-FAM ensures reliable signal generation across various bioconjugation applications.
Performance Highlights
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High-purity (≥ 99%) single isomer fluorescein dye
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Excitation/emission maxima: 493 nm / 517 nm
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Excellent fluorescence quantum yield and solubility
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Compatible with 488 nm laser sources
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Ideal for protein and nucleic acid labeling workflows
Properties
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Molecular Weight: 376.32
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Empirical Formula: C₂₁H₁₂O₇
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CAS Number: 3301-79-9
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Absorption [λmax]: 493 ± 3 nm
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Emission [λmax]: 517 ± 3 nm
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Extinction Coefficient (ε): >72,000 M⁻¹·cm⁻¹
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Excitation Source: 488 nm
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Reactive Moiety: Free carboxylic acid
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Solubility: Methanol, DMF, DMSO
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Buffer System for Fluorescence Readings: T&S Buffer, pH 9
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Appearance (form/color): Yellow-orange solid (powder)
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Storage Conditions: Store at room temperature. Keep dry and protect from light, especially in solution.
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Trade Names: 6-Carboxyfluorescein; 6-FAM; 6-Carboxyfluorescein, single isomer
Application
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Fluorescence labeling of biomolecules
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Bioconjugation of proteins and nucleic acids
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Confocal laser scanning microscopy
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Flow cytometry (FACS)
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Fluorescence imaging and detection systems
