Streamlined Affinity Purification for FPLC Workflows
Affinity
SMART Chromatography™ pre-packed columns bring efficiency and precision to FPLC-based affinity purification. Designed for clarified and non-clarified feed streams, these columns harness high-capacity agarose resins for selective capture of antibodies and His-tagged proteins. Optimized for compatibility with IMAC and Protein A workflows, SMART columns combine rapid processing with reproducible performance in antibody purification and recombinant protein isolation.
The following formats are available for FPLC purification:
Ion Exchange – A widely used method based on surface charge
Hydrophobic Interaction – Enables gentle purification under high-salt conditions
SMIRF™ – FPLC Screening Kit – For rapid evaluation of ligands and conditions
ZetaSep Ni-NTA FF6
FPLC column packed with Ni-NTA Agarose Resin. A cross-linked 6% beaded agarose IMAC resin for His-tagged protein purification.
ZetaSep NTA (metal-free) FF6
FPLC column packed with NTA Agarose Resin. A cross-linked 6% beaded agarose IMAC resin for His-tagged protein purification.
ZetaSep Protein A Elevate
FPLC column packed with NaOH-tolerant recombinant Protein A Agarose Resin. A cross-linked beaded agarose resin to purify immunoglobulins via their selective affinity to Protein A.