Description
High-Performance IMAC for His-Tagged Protein Purification
ZetaSep Ni-NTA FF6 from emp BIOTECH is a pre-packed FPLC column filled with 6% cross-linked agarose resin functionalized with nickel-charged nitrilotriacetic acid (Ni-NTA) ligands. Designed for immobilized metal affinity chromatography (IMAC), this column delivers selective purification of polyhistidine-tagged (6xHis) proteins under native or denaturing conditions.
The resin’s ~100 µm bead size ensures excellent flow characteristics, minimal backpressure, and reproducible performance across preparative workflows. ZetaSep Ni-NTA FF6 enables high-yield capture with efficient removal of impurities—even in challenging buffer systems or high-throughput conditions.
Performance Highlights
- ≥ 20 µmol Ni²⁺/mL resin ion density
- Average bead diameter ~100 µm
- Operating pressure up to 3 bar
- pH stability: 3–13
- Recommended flow rate: 0.5–10 mL/min (60–300 cm/h)
- Compatible with imidazole or low-pH elution
Specifications
- Resin: Zetarose Ni-NTA FF6
- Matrix: 6% cross-linked agarose
- Column housing: Polypropylene with 20 µm frits and 10/32 fittings
- Storage buffer: 20% ethanol
Applications
- IMAC-based purification of recombinant His-tagged proteins
- Native or denaturing condition workflows
- FPLC-based preparative protein purification
Why Choose ZetaSep Ni-NTA FF6?
ZetaSep Ni-NTA FF6 combines high selectivity for 6xHis-tagged proteins with robust pressure and chemical stability, making it ideal for reproducible, preparative IMAC workflows. Its column format supports seamless integration into FPLC systems for fast, scalable, and efficient affinity purification.
