Description
Reliable IMAC Affinity Resin for His-Tagged Protein Purification
ZetaSep NTA (metal-free) FF6 from emp BIOTECH is a 6% cross-linked beaded agarose resin functionalized with uncharged nitrilotriacetic acid (NTA) ligands for immobilized metal affinity chromatography (IMAC). Supplied as an FPLC column, it enables customizable purification of His-tagged proteins by charging the resin with your metal ion of choice (Ni²⁺, Co²⁺, Cu²⁺, Zn²⁺).
The resin binds polyhistidine-tagged proteins via selective affinity to chelated metal ions coordinated within the NTA ligand. After washing, proteins are eluted under native or denaturing conditions using imidazole, histidine, or low pH buffers. ZetaSep NTA FF6 offers flexibility and scalability from research to process development.
Performance Highlights
- Versatile binding: Chargeable with Ni²⁺, Co²⁺, Cu²⁺, or Zn²⁺ for tailored selectivity
- Robust support: Based on 6% cross-linked agarose with excellent pressure and chemical stability
- Precise particle range: 100 µm average bead diameter, ideal for FPLC applications
- High performance: ≥ 500 cm/h linear velocity with ≤ 3 bar pressure resistance
- Flexible elution: Compatible with imidazole, histidine, or pH shift strategies
Specifications
- Matrix: 6% highly cross-linked beaded agarose
- Functional Ligand: Uncharged NTA (metal-free)
- Average Bead Diameter: 100 µm
- Column Volume: 5 mL
- Column Height: 25 mm
Applications
- Immobilized metal affinity chromatography (IMAC)
- Purification of recombinant His-tagged proteins
- Elution under native or denaturing conditions
Why Choose ZetaSep NTA (metal-free) FF6?
ZetaSep NTA (metal-free) FF6 offers unmatched flexibility for IMAC workflows requiring metal selection control. Its consistent bead size and pressure-flow resilience make it ideal for high-purity capture of His-tagged proteins with customizable selectivity. Designed for reproducibility and ease of use in FPLC formats, it is a smart choice for method development and scale-up in protein purification.
