Description
Robust Affinity Purification of Antibodies with NaOH-Stable Protein A Resin
ZetaSep Protein A Elevate from emp BIOTECH is a pre-packed FPLC column designed for efficient and selective purification of immunoglobulins. It contains Zetarose Protein A Elevate—cross-linked beaded agarose functionalized with a NaOH-tolerant recombinant Protein A ligand. This robust resin enables consistent binding performance, even during repeated cleaning cycles with 1 M NaOH.
With an average bead diameter of ~100 µm, the column provides excellent flow properties and uniform packing for high-resolution purification. Its high binding capacity (≥ 40 mg human IgG/mL resin) makes it ideal for capturing antibodies directly from clarified protein A eluates or complex biological samples, without sacrificing throughput or reproducibility.
Performance Highlights
- High binding capacity: ≥ 40 mg human IgG/mL resin
- Tolerates 1 M NaOH exposure for ≥ 15 min
- Broad pH stability: 2–14 (short term), 3–9 (long term)
- Excellent performance under flow rates up to 10 mL/min
- Operates at pressures up to 3 bar
- Pre-packed in polypropylene FPLC column housing with 20 µm frits
Specifications
- Resin: Zetarose Protein A Elevate
- Matrix: Cross-linked 6% agarose, ~100 µm bead diameter
- Column volume: 1 mL or 5 mL (depending on format)
- Storage buffer: 20% ethanol
Applications
- Purification of monoclonal and polyclonal IgG
- Capture of antibodies from clarified harvests or protein A eluates
- High-yield workflows requiring repeated CIP steps
- FPLC-compatible affinity chromatography
Why Choose ZetaSep Protein A Elevate?
ZetaSep Protein A Elevate combines high antibody-binding capacity with base stability and reproducible performance, even in NaOH-driven CIP protocols. Its consistent flow properties and robust bead matrix make it a reliable tool for researchers seeking purity, recovery, and efficiency across repeated runs in automated or manual purification setups.
