Hydrophobic Interaction Columns for Optimized Protein Separation
Hydrophobic Interaction
Hydrophobic interaction chromatography (HIC) separates proteins based on surface hydrophobicity, offering a gentle and effective method for intermediate purification. SMART Chromatography™ HIC columns are pre-packed with butyl or phenyl ligands on large-bead agarose resins, optimized for reliable separation and scale-up in clarified or non-clarified feed streams.
Alternative SMART Chromatography™ formats may offer better alignment with specific purification requirements:
Ion Exchange Columns
Affinity Columns
SMART Chromatography™ Butyl
SMART Chromatography™ Butyl is a column packed with large diameter beaded agarose resin immobilized with butyl ligand. Specifically designed for SMART Chromatography™ hydrophobic interaction exchange separation.
SMART Chromatography™ Phenyl
SMART Chromatography™ Phenyl is a column packed with large diameter beaded agarose resin immobilized with phenyl ligand. Specifically designed for SMART Chromatography™ hydrophobic interaction exchange separation.