Fluorescent Dyes for Bioconjugation and Detection Applications
Fluorescent Dyes: Excitation 600–700 nm
Orange and red fluorophores for advanced labeling applications
This range features non-activated and amine-reactive dyes like TAMRA, ROX, and JOE, with excitation in the 500–600 nm range. These dyes are ideal for bioconjugation, FRET, and fluorescence detection in imaging and flow cytometry.
Their bright signal and spectral properties make them well-suited for multiplexing and high-sensitivity assays.
For other spectral options, see also:
Excitation 350–500 nm – Green-fluorescent dyes including fluorescein and FITC derivatives
Excitation 500–600 nm – Orange and red-fluorescent rhodamines and TAMRA-based labels
Excitation 600–700 nm
5/6-Carboxyfluorescein NHS ester, mixed isomers (5/6-FAM-SE)
Amine-reactive fluorescein dye marker for labeling of biomolecules (Abs: 496 nm, Em: 517 nm).
5/6-Carboxytetramethylrhodamine NHS ester, mixed isomers (5/6-TAMRA-SE)
Amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm).