Phosphoramidite Coupling Activators for Oligonucleotide Synthesis
Activators
Emp BIOTECH offers a dependable range of phosphoramidite coupling activators – DCI, ETT, and BMT – available in both crystalline and pre-mixed acetonitrile solutions. These activators are used in Step 2 of the oligonucleotide synthesis cycle and support efficient and reproducible coupling across a wide range of synthesis platforms. Suitable for DNA and RNA synthesis, they ensure low n–1 rates and help optimize synthesis time and phosphoramidite consumption. All formats are compatible with automated synthesis systems.
5-Benzylthiotetratzole (BTT) Crystal
5-Benzylthiotetrazole (BTT) Crystal, CAS 21871-47-6, is a high-performance activator used in automated DNA and RNA oligonucleotide synthesis to enable efficient phosphoramidite coupling with high yields and low n–1 impurity levels.
0.6 M 5-Ethylthiotetrazole (ETT) in anhydrous Acetonitrile
0.6 M 5-Ethylthiotetrazole (ETT) in anhydrous acetonitrile is a high-efficiency activator for phosphoramidite coupling in DNA and RNA oligonucleotide synthesis.
ETT Crystal (5-Ethylthiotetrazole)
ETT Crystal (5-Ethylthiotetrazole, CAS 89797-68-2) is used as a high-efficiency activator for phosphoramidite coupling in automated DNA and RNA oligonucleotide synthesis.
0.5 M 5-Ethylthiotetrazole (ETT) in anhydrous Acetonitrile
0.5 M 5-Ethylthiotetrazole (ETT) in anhydrous acetonitrile is a high-efficiency activator for phosphoramidite coupling in automated DNA and RNA oligonucleotide synthesis. It ensures fast reaction kinetics, high coupling yields, and minimal n–1 impurities—ideal for use with TBDMS, TOM®, and ACE® amidites across a wide range of synthesis platforms.
0.5 M 4,5-Dicyanoimidazole (DCI) in anhydrous Acetonitrile
4,5-Dicyanoimidazole (DCI) is an efficient activator for use in chemical synthesis of DNA.
1.0 M DCI Activator with NMI
4,5-Dicyanoimidazole (DCI) is an efficient activator for use in chemical synthesis of DNA.