High-Performance Anion Exchange for Scalable Biomolecule Purification
Zetarene Polymer Solid Phase: Robust Ion Exchange for Scalable Biomolecule Purification
Zetarene™ Q15 and Q30 are strong anion exchange resins designed for high-resolution purification of negatively charged biomolecules. Based on a highly crosslinked polystyrene/divinylbenzene matrix and functionalized with quaternary ammonium groups, these resins combine mechanical robustness, chemical resistance, and reliable scalability—making them ideal for both research and industrial purification workflows.
Performance Profiles
|
Feature |
Zetarene Q15 |
Zetarene Q30 |
|---|---|---|
|
Product Number |
TM-8201 |
TM-8202 |
|
Bead Size |
~15 µm (fine) |
~30 µm (standard) |
|
Ion Exchange Type |
Strong AEX (quaternary ammonium) |
Strong AEX (quaternary ammonium) |
|
Matrix |
Crosslinked PS-DVB |
Crosslinked PS-DVB |
|
Ionic Capacity |
≥ 0.08 mmol Cl⁻ / mL resin |
≥ 0.08 mmol Cl⁻ / mL resin |
|
pH Stability |
pH 2–12 |
pH 2–12 |
|
Max Linear Flow Rate |
≥ 1800 cm/h |
≥ 1800 cm/h |
|
Pressure Tolerance |
High |
High |
|
Typical Use Case |
High-resolution polishing of oligonucleotides and nucleic acids |
Preparative and production-scale oligonucleotide, protein and enzyme purification |
Applications
Zetarene™ Q resins are used across a range of purification tasks where strong anion exchange chromatography is required. Their versatility and robustness make them a reliable choice in:
Oligonucleotide purification and polishing: Remove truncated sequences with high resolution.
Nucleic acid cleanup: Effective for plasmid DNA, RNA, or genomic DNA under varying buffer conditions.
Protein and enzyme purification: Ideal for intermediate and final purification steps.
Bioprocessing workflows: Suitable for both lab-scale screening and large-scale GMP manufacturing.
Performance Highlights
Ultra-sharp resolution: Narrow particle size distributions minimize band broadening and maximize separation efficiency.
Low backpressure design: Achieve high flow rates without compromising resolution.
Robust chemical and mechanical stability: Compatible with aggressive cleaning-in-place protocols including sodium hydroxide.
Consistent performance at scale: Identical bead chemistry across formats ensures seamless scale-up and reproducibility.
pH stability range: Broad pH tolerance from 2 to 12 enables flexible method development and cleaning cycles.