Description
Scalable Hydrophobic Interaction Chromatography for Protein Separation
ZetaCell Phenyl from emp BIOTECH is a cross-linked agarose-based resin designed for SMART™ HIC (Hydrophobic Interaction Chromatography) applications. Functionalized with a phenyl ligand (–C₆H₅), this robust resin enables scalable and selective purification of proteins and other biomolecules based on hydrophobic interactions. The large bead diameter (250 µm) ensures fast flow rates and compatibility with SMART radial-flow formats, even from unclarified feed streams.
ZetaCell Phenyl is part of emp BIOTECH’s next-generation SMART chromatography portfolio, which eliminates the need for clarification steps and allows direct capture from complex feed materials—accelerating workflows and maximizing protein recovery.
Performance Highlights
- Ligand: Phenyl (–C₆H₅)
- Matrix: Cross-linked agarose
- Particle size: 250 µm
- Modality: Hydrophobic interaction chromatography (HIC)
- SMART™ compatible
Specifications
- Resin: ZetaCell Phenyl
- Support: Beaded agarose, cross-linked
- Bead size: 250 µm (large-diameter for high flow)
- Application: SMART™ HIC
- Storage: 20% ethanol
- Autoclavable: 121 °C for 20 min
- Use: Gravity or packed-bed chromatography
Applications
- HIC-based intermediate or polishing steps
- Capture of hydrophobic proteins from clarified and unclarified feeds
- Direct processing from mammalian, yeast, or microbial cultures
Why Choose ZetaCell Phenyl?
ZetaCell Phenyl enables scalable, high-throughput HIC separations—especially suited for SMART™ workflows that integrate clarification and purification in one step. Its robust agarose matrix and optimized phenyl ligand density ensure reliable performance across a wide range of protein purifications.
