Description
High-Performance Antibody Purification with NaOH-Stable Protein A Resin
ZetaCell Protein A Elevate from emp BIOTECH is a robust affinity chromatography resin designed for simplified, efficient antibody capture using SMART™ Chromatography. This base-stable, large-diameter beaded agarose resin is surface-modified with a NaOH-tolerant recombinant Protein A ligand, enabling direct processing of cell-containing feedstocks in bioprocessing and continuous manufacturing workflows.
The 250 µm beads are highly cross-linked to prevent clogging and channeling, even when challenged with cell debris or lysate. ZetaCell Protein A Elevate maintains structural integrity under alkaline conditions, supports extended cleaning-in-place (CIP) cycles, and allows consistent performance over multiple runs. It is ideal for immunoglobulin purification in applications that prioritize process efficiency, resin longevity, and uncluttered flow dynamics over maximum binding capacity.
Performance Highlights
- ≥ 30 mg/mL binding capacity (human IgG)
- ≥ 80% particles in the 140–360 µm range
- Linear velocity ≥ 2000 cm/h
- Stable in 1 M NaOH for ≥ 15 minutes
- Operates efficiently at up to 3 bar system pressure
- NaOH-tolerant recombinant Protein A on cross-linked 250 µm agarose beads
Specifications
- Matrix: Highly cross-linked beaded agarose
- Ligand: Recombinant NaOH-tolerant Protein A
- Bead Diameter: Average 250 µm
- pH Stability: 2–14 (short term), 3–9 (long term)
- Storage: +2 to +8 °C in 20% ethanol
Applications
- Immunoglobulin G (IgG) and monoclonal antibody purification
- Continuous capture in perfusion bioprocessing
- Capture of antibodies from clarified or unclarified feeds
- Alkaline-tolerant workflows with repeated CIP
Why Choose ZetaCell Protein A Elevate in a SMART™ column?
ZetaCell Protein A Elevate combines efficiency with base stability and clog-resistant bead geometry to simplify antibody capture while maximizing throughput and resin lifetime. Its design enables effective operation in SMART™ columns without relying on high binding capacity, making it ideal for continuous or perfusion-based workflows where speed, robustness, and process compatibility are key.
