Description
Efficient SMART™ IEX with Surface-Enhanced Weak Anion Exchange Resin
ZetaCell DEAE Boost from emp BIOTECH is a large-bead weak anion exchange agarose resin tailored for SMART™ ion exchange chromatography. The solid phase is composed of highly cross-linked beaded agarose derivatized with diethylaminoethyl (DEAE) ligands, strategically localized at the bead surface to enhance ion exchange availability. This surface-enhanced design increases binding efficiency while maintaining excellent flow dynamics.
The average 250 µm bead size, with ≥ 80% in the 140–360 µm range, enables unhindered passage of cells and debris through the packed bed, making it ideal for clarified or unclarified feedstocks in continuous bioprocessing. ZetaCell DEAE Boost is chemically stable across a broad pH range and supports fast, scalable separations with linear flow rates ≥ 2000 cm/h.
Performance Highlights
- Enhanced surface density of weak anion ligands for improved binding
- ≥ 80% of beads in the 140–360 µm range
- Linear velocity ≥ 2000 cm/h
- Operates at pressures up to 3 bar
- pH stability: 2–14 (short term), 3–12 (long term)
- Cross-linked, hydrophilic agarose beads designed for SMART™ Chromatography
Specifications
- Matrix: Cross-linked beaded agarose
- Ligand: Diethylaminoethyl (DEAE), weak anion exchanger
- Bead Diameter: Average 250 µm
- Ion Exchange Capacity: 0.08–0.22 mmol Cl⁻/mL resin
- Storage Buffer: 20% ethanol
Applications
- Weak anion exchange (AEX) chromatography
- Purification of acidic proteins, DNA, and other negatively charged biomolecules
- Continuous or batch ion exchange workflows
- SMART™ chromatography with cell-containing or clarified feeds
Why Choose ZetaCell Q Boost?
etaCell DEAE Boost delivers improved binding capacity and operational efficiency through surface-localized ion exchange functionality. Optimized for SMART™ column geometry, it supports high-speed purification and reliable performance with cell-rich samples—ideal for labs and manufacturers seeking scalable, high-throughput IEX with reduced fouling and robust pH tolerance.
